Enhancing Root Development: The Impact of Indole-3-Butyric Acid on Nodal Leaf and Tip Cuttings of Dendrocalamus longispathus and Bambusa tulda
DOI:
https://doi.org/10.56946/jspae.v3i2.408Keywords:
Nodal leaf cuttings, tip cuttings, IBA, Principal Component analysis, branch cuttingsAbstract
This study examines the effects of two propagation techniques—nodal leaf cuttings and tip cuttings—on root development in two bamboo species, Dendrocalamus longispathus and Bambusa tulda. The experiment aimed to determine the most effective propagation method for enhancing root growth, addressing the challenge of optimizing bamboo cultivation for both conservation and commercial purposes. The experiment was conducted using a non-mist propagator system, under controlled humidity and temperature, to observe differences in root number and length after four weeks. Our results indicate significant differences between the two cutting methods across both species, with D. longispathus generally demonstrating more substantial root growth compared to B. tulda in both metrics. Specifically, for D. longispathus, nodal leaf cuttings showed a consistently higher mean root length, whereas for B. tulda, tip cuttings eventually resulted in slightly longer roots, highlighting a species-specific response to the propagation methods. Statistical analysis confirmed significant differences (p<0.05) in root growth dynamics between treatments, underscoring the importance of choosing appropriate propagation techniques based on species-specific responses. This research contributes to the understanding of bamboo propagation strategies and suggests further exploration into species-specific cultivation methods to enhance growth and conservation efforts.
References
Alamgir, M., Mezbahuddin, M., Jashimuddin, M. Role of bamboo-based cottage industry in economic upliftment of rural poor of Chittagong, Bangladesh. Journal of Bamboo and Rattan. (2007). 6(3–4), 157–164.
Chowdhury, M. I. H., & Chinmoy Das. Carbon Sequestration Potentials for Conservation of Sheikh Russel Aviary and Eco-Park, Rangunia, Chittagong. Journal of Environmental Science and Economics. (2024). 3(2), 90–101. https://doi.org/10.56556/jescae.v3i2.940
Das, G., Kim, D.Y., Fan, C., Gutiérrez-Grijalva, E.P., Heredia, J.B., Nissapatorn, V., Mitsuwan, W., Pereira, M.L., Nawaz, M., Siyadatpanah, A., Norouzi, R., Sawicka, B., Shin, H.S., Patra, J.K. Plants of the genus Terminalia: An insight on its biological potentials, pre-clinical and clinical studies. Frontiers in Pharmacology, 11(October). (2020). 1–30. https://doi.org/10.3389/fphar.2020.561248
El-Keltawi, E.M., Abdel-Rahman, S.A. Propagation of Bamboo (Dendrocalamus giganteus, Munro) through culm-branch cuttings in Egypt. Assiut Journal of Agricultural Sciences. (2010). 41(1), 1–22. https://doi.org/10.21608/ajas.2010.267818
Goyal, A.K., Sen, A. In vitro regeneration of bamboos, the “Green Gold”: An overview. Indian Journal of Biotechnology. (2016). 15(1), 9–16.
Hamalton, T. Vegetative propagation techniques for bamboo species: A review. International Journal of Agriculture Environment and Biotechnology. (2022). 15(1), 101–107. https://doi.org/10.30954/0974-1712.01.2022.13
Hossain, M.A., Kumar, S.M., Seca, G., Maheran, A.A., Nor-Aini, A.S. Mass propagation of Dendrocalamus asper by branch cutting. Journal of Tropical Forest Science. (2018). 30(1), 82–88. https://doi.org/10.26525/jtfs2018.30.1.8288
Imran, M., Chowdhury, H., Rakib, M. H., & Das, C. (2023). Intra and Interspecific Relation of Tree Species Aspects of Seed Biometry, Phenology , Seed Dispersion , and Germination. Journal of Agriculture Sustainability and Environment, 2(2), 57–69. https://doi.org/https://doi.org/10.56556/jase.v2i2.877
Imran, M., Chowdhury, H., Rakib, M. H., Das, C., & Hossain, Z. (2024). Tree Species Germination : A Comprehensive Meta-Analysis and its Implications for Pre-Sowing Treatment in Bangladesh. Journal of Soil, Plant and Environment. 3(1), 24–40. https://doi.org/10.56946/jspae.v3i1.397
Ilorkar, V.M., Raut, P.D., Atram, K.P.C., P, K.A. Effect of growth hormones IBA on clonal propagation of bamboo in nursery condition. Journal of Pharmacognosy and Phytochemistry. (2021). 10(1), 369–370. www.phytojournal.com
Islam, M.S., Bhuiyan, M.K., Hossain, M.M., Hossain, M.A. Clonal propagation of Bambusa vulgaris by leafy branch cuttings. Journal of Forestry Research. (2011). 22(3), 387–392. https://doi.org/10.1007/s11676-011-0109-4
Jamatia, S. Livelihood of the bamboo base: Challenges and opportunities. Proceedings of the 55th International Convention of Society of Wood Science and Technology, Beijing, China. (2012). 1–16.
Jha, A., Das, S. Assessment of in-vitro culture through nodal explants of Dendrocalamus hamiltonii. International Journal of Agricultural and Applied Sciences. (2021).2(1), 130–133. https://doi.org/10.52804/ijaas2021.2115
Jha, A., Das, S., Kumar, B. Micropropagation of Dendrocalamus hamiltonii through nodal explants. Global Journal of Bio-Science and Biotechnology.(2013). 2(4), 580–582.
Kalanzi, F., Mwanja, C.K. Effect of nodal cutting position and plant growth regulator on bud sprouting of Dendrocalamus giganteus Wall. Ex Munro in Uganda. Advances in Bamboo Science. (2023). 2(January), 100016. https://doi.org/10.1016/j.bamboo.2023.100016
Kaushal, R., Gulabrao, Y.A., Tewari, S.K., Chaturvedi, S., Chaturvedi, O.P.Rooting behaviour and survival of bamboo species propagated through branch cuttings. Indian Journal of Soil Conservation, January. (2011).
Kumar Ahirwar, R., Bagchi, S.N.In vitro micropropagation of Dendrocalamus strictus: Review. International Journal of Genetic Engineering and Biotechnology, ISSN 0974. (2014).3073(1), 7–13. http://www.irphouse.com
Md Numan, S. Multipurpose uses of bamboo plants: A review. International Research Journal of Biological Sciences. (2015). 4(12), 57–60. https://www.researchgate.net/publication/287199703
Mishra, J.P., Bhadrawale, D., Rana, P.K., Mishra, Y. Evaluation of cutting diameter and hormones for clonal propagation of Bambusa balcooa Roxb. Journal of Forest Research. (2019) 24(5), 320–324. https://doi.org/10.1080/13416979.2019.1658699
Ojha, A., Verma, N., Kumar, A.In vitro micropropagation of economically important edible bamboo (Dendrocalamus asper) through somatic embryos from root, leaves and nodal segments explants. Research on Crops. (2009). 10(2), 430–436.
Pawar, S.V., Patil, S.C., Jambhale, V.M., Mehetre, S.S. Effect of growth regulators on in vitro multiplication of sugarcane varieties. Indian Sugar.(2002) 52(2), 109–111. http://cat.inist.fr/?aModele=afficheN&cpsidt=13888985
Rakib M.H., Chowdhury M.I.H., Das C., et al. Wildlife Ecological Spectrum: unveiling alpha (α), beta (β), and gamma (γ) diversity of the Kaptai National Park, Bangladesh, 24 July 2024, PREPRINT (Version 1) available at Research Square. https://doi.org/10.21203/rs.3.rs-4668666/v1
Ramanayake, S.M.S.D., Yakandawala, K. Micropropagation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms. Plant Science. (1997). 129(2), 213–223. https://doi.org/10.1016/S0168-9452(97)00185-4
Rout, G.R. Analysis of current methods and approaches on the micropropagation of bamboo. National Academy of Sciences, India, January. (2020). 235–246.
Sahoo, S., Kar, R.K., Mohanty, T.L. Effect of plant growth hormones on sprouting and rooting behaviour of clones of various bamboo species in coastal Odisha. International Journal of Current Microbiology and Applied Sciences. (2020). 9(7), 3521–3532. https://doi.org/10.20546/ijcmas.2020.907.411
Shirin, F., Mishra, J.P., Bhadrawale, D., Saudagar, I.A., Gupta, T., Berry, N. Seasonal and hormonal variation during adventitious rhizogenesis in five commercially important bamboo species for production of quality planting material. Journal of Forest Research.(2021). 26(5), 377–385. https://doi.org/10.1080/13416979.2021.1935548
Singh, K. Micropropagation of Bambusa vulgaris var. striata. Asian Journal of Bio Science. (2016).11(1), 127–130. https://doi.org/10.15740/has/ajbs/11.1/127-130
Singh, S.R., Dalal, S., Singh, R., Dhawan, A.K., Kalia, R.K. Micropropagation of Dendrocalamus asper (Schult. & Schult. f.) Backer ex K. Heyne: An exotic edible bamboo. Journal of Plant Biochemistry and Biotechnology. (2012) 21(2), 220–228. https://doi.org/10.1007/s13562-011-0095-9
Sohel, M.S.I., Alamgir, M., Akhter, S., Rahman, M. Carbon storage in a bamboo (Bambusa vulgaris) plantation in the degraded tropical forests: Implications for policy development. Land Use Policy, 49(February). (2015).142–151. https://doi.org/10.1016/j.landusepol.2015.07.011
Sood, A., Ahuja, P.S., Sharma, M., Sharma, O.P., Godbole, S. In vitro protocols and field performance of elites of an important bamboo Dendrocalamus hamiltonii Nees et Arn. ex Munro. Plant Cell, Tissue and Organ Culture.(2002). 71(1), 55–63. https://doi.org/10.1023/A:1016582732531
Suwal, M.M., Lamichhane, J., Gauchan, D.P. Regeneration technique of bamboo species through nodal segments: A review. Nepal Journal of Biotechnology. (2020). 8(1), 54–68. https://doi.org/10.3126/njb.v8i1.30209
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